Laboratory Info

The Laboratory for Genomics and Bioinformatics is a genomics facility offering DNA sequencing (custom and genome-scale projects), genotyping (microsatellite and SNP), microarray design, hybridization and data analysis, and other services.

Allison Gillaspy, Ph.D., is the Director of the Laboratory for Genomics and Bioinformatics and a Research Assistant Professor in the Department of Microbiology and Immunology.

 

 


Real Time PCR with the ABI 7500

The ABI 7500 Fast Real-Time PCR system has a new high-speed thermal-cycling block and new master mix formulations that allow the user to perform real-time PCR runs in less than 40 minutes. Variable excitation and five-color detection allow the use of several dyes, including FAM/SYBR Green I, VIC/JOE, NED/TAMRA/Cy3, ROX/Texas Red, and Cy5, for greater assay versatility. With this one machine, we can perform a variety of applications, including absolute and relative quantification, identify gene expression changes, detect and quantitate pathogens, and SNP genotyping. The presence or absence of transgenes and other target sequences also can be determined.

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