Protocols

The following protocols have been obtained and adapted from several sources including:

University of Oklahoma's Advanced Center for Genome Technology (OU-ACGT)
The Institute for Genomic Research (TIGR)

 

• ABI Prism 377 Protocols
• Big Dye Protocols and Notes - Plasmid Templates - (OU-ACGT)
• Double-stranded DNA Double Acetate Isolation - (OU-ACGT)
• Isolation of DNA Templates
• Oligonucleotide Synthesis Using the MerMade
• Shrimp Alkaline Phosphatase (SAP) and ExonucleaseI Treatment of PCR Products
• Sequential Injection Before Analysis (SIBA)
• Cleaning Up Reactions for the ABI 3700
• Making Cracking Gel Buffer
• Cracking Gel Procedure
• Pouring Plates with LB Agar
• NexPrep DNA Plasmid Preparation
• PCR
• Rehydrating Reactions for the ABI 3700
• Running the Hydra
• Running Reactions on the ABI 3700
• Setting Up Customer Reactions
• Setting Up Reactions on the Abi 3700
• Running the PF2D